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	Provedor de dados ArchiMer (2) Autor Aubry, T. (1) Claeys-bruno, M. (1) Duthoit, Frederique (1) Godfroy, Anne (1) Landreau, M. (1) Landreau, Matthieu (1) Le Blay, Gwenaelle (1) Vandenabeele-trambouze, O. (1) Mais... Palavra-chave Gellan (2) Xanthan (2) (hyper)thermophile (1) (hyper)thermophilic marine micro-organisms (1) Anaerobiosis (1) Cell immobilization (1) Continuous culture (1) Culture continue (1) Entrapment (1) Gellane (1) Immobilisation cellulaire (1) Immobilization (1) Rainbow site (1) Site Rainbow (1) Xanthane (1) Mais... Tipo do documento Text (2) Ano 2016 (2) País France (2) Idioma Francês (1) Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Entrapment of anaerobic thermophilic and hyperthermophilic marine microorganisms in a gellan/xanthan matrix ArchiMer Landreau, M.; Duthoit, Frederique; Claeys-bruno, M.; Vandenabeele-trambouze, O.; Aubry, T.; Godfroy, Anne; Le Blay, Gwenaelle. Aims The aims of this study were (i) to develop a protocol for the entrapment of anaerobic (hyper)thermophilic marine micro-organisms; (ii) to test the use of the chosen polymers in a range of physical and chemical conditions and (iii) to validate the method with batch cultures. Methods and Results The best conditions for immobilization were obtained at 80°C with gellan and xanthan gums. After 5-week incubation, beads showed a good resistance to all tested conditions except those simultaneously including high temperature (100°C), low NaCl (<0∙5 mol l−1) and extreme pH (4/8). To confirm the method efficiency, batch cultures with immobilized Thermosipho sp. strain AT1272 and Thermococcus kodakarensis strain KOD1 showed an absence of detrimental effect... Tipo: Text Palavras-chave: (hyper)thermophilic marine micro-organisms; Anaerobiosis; Entrapment; Gellan; Immobilization; Xanthan. Ano: 2016 URL: http://archimer.ifremer.fr/doc/00332/44352/44000.pdf Immobilisation et culture continue en bioréacteur gas-lift de microorganismes marins thermophiles et hyperthermophiles anaérobies ArchiMer Landreau, Matthieu. Since the discovery of hydrothermal vents, multiple studies have been conducted in order to study microbial diversity. Molecular inventories realized have thus demonstrated a great diversity of species that contrasts with the low proportion (1%) of species isolated by culture approach. A new cell immobilization approach by inclusion in a polymer matrix (gellan and xanthan) has been developed for the study of these thermophilic anaerobic marine communities. The system, based on the formation of an emulsion between an inoculated polymer solution and oil, allows the entrapment of cells in gel beads with a diameter between 1 and 2 mm. The optimal immobilization conditions were obtained for emulsion performed at 80 °C with stirring (150 rpm) with a polymer... Tipo: Text Palavras-chave: Immobilisation cellulaire; Gellane; Xanthane; Site Rainbow; Culture continue; Cell immobilization; Gellan; Xanthan; Rainbow site; (hyper)thermophile; Continuous culture. Ano: 2016 URL: https://archimer.ifremer.fr/doc/00498/60933/64327.pdf Registros recuperados: 2 Primeira ... 1 ... Última

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